Functional differentiation of spider hemocytes by light and transmission electron microscopy, and MALDI-MS-imaging

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• 作者：Lucia Kuhn-Nentwig ; Lukas S. Kopp ; Wolfgang Nentwig ; Beat Haenni ; Kathrin Streitberger ; Stefan Sch眉rch ; Johann Schaller
• 关键词：Cupiennius salei ; MALDI-IMS ; Ctenidins ; SIBD-1 ; Defensin ; Innate immune system
• 刊名：Developmental and Comparative Immunology
• 出版年：March, 2014
• 年：2014
• 卷：43
• 期：1
• 页码：59-67
• 全文大小：1767 K

文摘

The most abundant cell types in the hemolymph of Cupiennius salei are plasmatocytes (70-80%) and granulocytes (20-30%). Both cells differ in shape, cytochemical and transmission electron microscopy staining of their cytoplasma and granules. According to MALDI-IMS (matrix-assisted laser desorption ionisation-mass spectrometry imaging), granulocytes exhibit ctenidin 1 (9510 Da) and ctenidin 3 (9568 Da), SIBD-1 (8675 Da), and unknown peptides with masses of 2207 and 6239 Da. Plasmatocytes exhibit mainly a mass of 6908 Da. Unknown peptides with masses of 1546 and 1960 Da were detected in plasmatocytes and granulocytes. Transmission electron microscopy confirms the presence of two compounds in one granule and cytochemical staining (light microscopy) tends to support this view. Two further hemocyte types (cyanocytes containing hemocyanin and prehemocytes as stem cells) are only rarely detected in the hemolymph. These four hemocyte types constitute the cellular part of the spider immune system and this is discussed in view of arachnid hemocyte evolution.