- 作者：Yingchun Han ; Edward R ; ell ; Sudesh Vasdev ; Vicki Gill ; Matthew Curran ; Leigh Anne Newhook ; Marie Grant ; Donna Hagerty ; Celine Schneider
- 关键词：Advanced glycation end product ; Type 1 diabetes ; Hemoglobin A1C ; Methylglyoxal ; Methylglyoxal-derived hydroimidazolone ; Mass spectrometry ; Solid phase extraction
- 刊名：Clinical Biochemistry
- 出版年：2009
- 出版时间：May 2009
- 年：2009
- 卷：42
- 期：7-8
- 页码：562-569
- 全文大小：472 K
ObjectivesElevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-free young individuals with Type 1 diabetes (T1DM). The relationship of plasma free MG-H to hemoglobin A1C (A1C) and plasma methylglyoxal levels was also determined.Design and methods
A solid phase extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed, and free plasma MG-H levels were measured in 40 T1DM patients (DM group), aged 6–21 years, and 11 non-diabetics (ND group), 6–22 years. Methylglyoxal was measured using LC-MS/MS and A1C by a Tosoh G7 high-performance liquid chromatograph.
Results
Our method showed high recovery, sensitivity and short run-time. Plasma free MG-H (nmol/L) was higher (p < 0.001) in the DM group (1318 ± 569; mean ± standard deviation) as compared to the ND group (583 ± 419). Within the DM group, plasma free MG-H did not correlate with plasma methylglyoxal or A1C.
Conclusions
Our LC-MS/MS method to measure free MG-H in plasma may be useful for future clinical application. The increased levels of free MG-H observed in individuals with TIDM are not merely the result of short term changes in glucose or methylglyoxal, but may reflect long-term alterations to tissue proteins.