Flow injection chemiluminescence determination of isoniazid using luminol and silver nanoparticles

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• 作者：Behzad Haghighi ; Somayyeh Bozorgzadeh
• 关键词：Chemiluminescence ; Isoniazid ; Silver nanoparticle ; Luminol ; Flow injection analysis
• 刊名：Microchemical Journal
• 出版年：2010
• 出版时间：July 2010
• 年：2010
• 卷：95
• 期：2
• 页码：192-197
• 全文大小：625 K

文摘

The effect of silver colloidal nanoparticles (AgNPs) on the luminol–isoniazid system was investigated. It was found that AgNPs could act as a nanocatalyst on the luminol–isoniazid system to generate chemiluminescence (CL). The CL emission spectrum of the luminol–isoniazid–AgNPs system showed a peak with a maximum at 425 nm. It was suggested that the luminophor species was the excited state 3-aminophthalate. The reduction of dissolved O₂ to H₂O₂ by isoniazid and decomposition of H₂O₂ to the oxygen-related radicals were attributed to the catalytic effect of AgNPs. Under optimized conditions, the CL signal intensity was linear with the isoniazid concentration in the range of 10–1000 ng mL^− 1, with the correlation coefficient of 0.9996. The limit of detection was 2.7 ng mL^− 1 isoniazid. The relative standard deviations for seven repeated measurements of 60 and 200 ng mL^− 1 isoniazid were 1.4 and 2.4 % , respectively. The effect of potent interfering compounds on the CL signal intensity of the proposed luminol–isoniazid–AgNPs system was investigated. The proposed method was successfully applied to the determination of isoniazid in a pharmaceutical sample.