Fabrication of a highly sensitive electrochemiluminescence lactate biosensor using ZnO nanoparticles decorated multiwalled carbon nanotubes

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• 作者：Behzad  ; Haghighi  ; ;   ; ^{haghighi@iasbs.ac.ir} ; Somayyeh  ; Bozorgzadeh
• 关键词：Electrochemiluminescence ; ZnO nanoparticles ; Multiwalled carbon nanotubes ; Luminol ; Lactate biosensor
• 刊名：Talanta
• 出版年：2011
• 出版时间：30 September 2011
• 年：2011
• 卷：85
• 期：4
• 页码：2189-2193
• 全文大小：412 K

文摘

ZnO nanoparticles (nanoZnO) were decorated on multiwalled carbon nanotubes (MWCNTs) and then the prepared nano-hybrids, nanoZnO-MWCNTs, were immobilized on the surface of a glassy carbon electrode (GCE) to fabricate nanoZnO-MWCNTs modified GCE. The prepared electrode, GCE/nanoZnO-MWCNTs, showed excellent electrocatalytic activity towards luminol electrochemiluminescence (ECL) reaction. The electrode was then further modified with lactate oxidase and Nafion to fabricate a highly sensitive ECL lactate biosensor. Two linear dynamic ranges of 0.01?0 ¦Ìmol L^? and 10?00 ¦Ìmol L^? were obtained for lactate with the correlation coefficient better than 0.9996. The detection limit (S/N = 3) was 4 nmol L^? lactate. The relative standard deviation for repetitive measurements (n = 6) of 10 ¦Ìmol L^? lactate was 1.5 % . The fabrication reproducibility for five biosensors prepared and used in different days was 7.4 % . The proposed ECL lactate biosensor was used for determination of lactate in human blood plasma samples with satisfactory results.