Direct electron transfer from glucose oxidase immobilized on a nano-porous glassy carbon electrode

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• 作者：Behzad  ; Haghighi  ; ;   ; ^{haghighi@iasbs.ac.ir} ; Mahmoud Amouzadeh  ; Tabrizi
• 关键词：Nano-porous glassy carbon electrode ; Activated glassy carbon electrode ; Direct electron transfer ; Glucose oxidase ; Glucose biosensor
• 刊名：Electrochimica Acta
• 出版年：2011
• 出版时间：30 November 2011
• 年：2011
• 卷：56
• 期：27
• 页码：10101-10106
• 全文大小：795 K

文摘

A pair of well-defined and reversible redox peaks was observed for the direct electron transfer (DET) reaction of an immobilized glucose oxidase (GOx) on the surface of a nano-porous glassy carbon electrode at the formal potential (E¡ã? of ?.439 V versus Ag/AgCl/saturated KCl. The electron transfer rate constant (k_s) was calculated to be 5.27 s^?. The dependence of E¡ã?on pH indicated that the direct electron transfer of the GOx was a two-electron transfer process, coupled with two-proton transfer. The results clearly demonstrate that the nano-porous glassy carbon electrode is a cost-effective and ready-to-use scaffold for the fabrication of a glucose biosensor.