Spiro-configured fluorescent probe: Synthesis and applications in the determinations of Hg2+ and proton, and two-photon fluorescence imaging
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文摘

We report a Hg2+ two-photon fluorescent probe based on spirobifluorene for the first time.

The molecule exhibits high selectivity and excellent sensitivity in both UV/Vis absorbance and one-photon fluorescence detection of Hg2+.

The molecule is a pH probe and suitable to detect acidic regions in cells.

The two-photon emission wavelength change before and after addition of Hg2+ is large.

The molecule enables fluorescence imaging of both the probe and mercury ions in cells by two-photon microscopy.

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