The Protective Role of Procyanidins and Lycopene Against Mercuric Chloride Renal Damage in Rats

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• 作者：HaiBo  ; YANG ; ZhaoFa  ; XU  ; ;   ; ^{zfxu@mail.cmu.edu.cn} ; Wei  ; LIU ; Yu  ; DENG ; Bin  ; XU[Author vitae]
• 关键词：Mercuric chloride ; Procyanidins ; Lycopene ; Renal damage ; Protection
• 刊名：Biomedical and Environmental Sciences
• 出版年：2011
• 出版时间：October 2011
• 年：2011
• 卷：24
• 期：5
• 页码：550-559
• 全文大小：1699 K

文摘

Objective

This study aims to investigate the protection of procyanidins and lycopene from the renal damage induced by mercuric chloride.

Methods

Rats were treated with either procyanidins or lycopene 2h before HgCl₂ subcutaneously injection, once daily treatment for 2 successive days.

Results

In comparison with HgCl₂ group, markers of renal function such as blood urea nitrogen in serum and urinary protein were decreased to (18.45¡À11.63) mmol/L and (15.93¡À9.36) mmol/L, (4.54¡À0.78) g/(g¡¤Cr) and (4.40¡À1.12) g/(g¡¤Cr). N-acetyl-beta-D-glucosaminidase, lactate dehydrogenase, alkaline phosphatase in urine were depressed to (125.49¡À11.68) U/(g¡¤Cr), (103.73¡À21.79) U/(g¡¤Cr), (101.99¡À12.28) U/(g¡¤Cr), and (113.19¡À23.74) U/(g¡¤Cr), (71.14¡À21.80) U/(g¡¤Cr), (73.64¡À21.51) U/(g¡¤Cr) in procyanidins and lycopene groups. Indicators of oxidative stress, for example, Glutathion was reduced to (45.58¡À9.89) ¦Ìmol/(g¡¤pro) and (45.33¡À5.90) ¦Ìmol/(g¡¤pro), and antioxidant enzymes such as superoxide dismutase, glutathione-peroxidase were enhanced to (43.07¡À10.97) U/(mg¡¤pro) and (39.94¡À6.04) U/(mg¡¤pro), (83.85¡À18.48) U/(mg¡¤pro), and (85.62¡À12.68) U/(mg¡¤pro). Malondialdehyde was lowered to (0.95¡À0.12) (¦Ìmol/g¡¤pro) and (1.03¡À0.12) ¦Ìmol/(g¡¤pro) in procyanidins and lycopene groups. ROS generation was decreased by 27.63 % and 16.40 % and apoptosis was also decreased in procyanidins and lycopene groups respectively. Pathological changes were much better as well.

Conclusion

Procyanidins and Lycopene play some protective role against mercury kidney damage.