Enhancement of soluble expression of codon-optimized Thermomicrobium roseum sarcosine oxidase in Escherichia coli via chaperone co-expression
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- 作者：Yanjun Tonga ; b ; 1 ; Shoushuai Fenga ; 1 ; fengss@jiangnan.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yu Xina ; Hailin Yanga ; Ling Zhanga ; Wu Wanga ; bioprocessor@aliyun.com" class="auth_mail" title="E-mail the corresponding author ; Wei Chenb
- 关键词：Thermomicrobium roseum ; Sarcosine oxidase ; E.coli ; Co-expression of chaperones ; Soluble expression
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 January 2016
- 年：2016
- 卷：218
- 期：Complete
- 页码：75-84
- 全文大小：4433 K
文摘
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Based on whole-genome analysis of T. roseum DSM 5159, the codon-optimized sox was successfully expressed in E. coli.
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The soluble expression of SOX was significantly enhanced via co-expression of chaperones (GroES-GroEL and DnaK-DnaJ-GrpE-GroES-GroEL).
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Analysis of intermolecular forces indicated that Arg, hydrogen bonds, and ionic bonds enhanced the interactions and stability of the TrSOX secondary structures.