- 作者：Feng Zhang ; Liquan Tong ; Haiquan Qiao ; Xuesong Dong ; Guojun Qiao ; Hongchi Jiang ; Xueying Sun
- 关键词：taurine ; intestinal ischemia reperfusion ; intestine ; liver ; kidney ; lung ; apoptosis ; lipid peroxide ; tumor necrosis factor-α ; caspase-3
- 刊名：Journal of Surgical Research
- 出版年：2008
- 出版时间：September 2008
- 年：2008
- 卷：149
- 期：1
- 页码：101-109
- 全文大小：3629 K
Background
Intestinal ischemia reperfusion (II/R) is a serious clinical condition associated with simultaneous multiple organ dysfunction. The purpose of this study was to investigate whether taurine, an anti-oxidative, anti-inflammatory, and anti-apoptotic agent, could attenuate multiple organ injury induced by II/R.Materials and methods
Taurine was intravenously injected to Wistar rats 30 min before II/R; physiological saline and sham operation served as controls. II/R was produced by occlusion of superior mesenteric artery for 60 min. Rats were randomly sacrificed 1.5, 3, 12, and 36 h after II/R; blood samples were collected for assessing alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (Cr), and tumor necrosis factor-alpha (TNF-α), and intestines, livers, kidneys, and lungs were removed for histological examination of scoring injury severity and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling analysis. The amount of lipid peroxides (LPO) was measured in intestinal tissues, and expression of caspase-3 was detected in all of the tissues with Western blot analysis.
Results
II/R resulted in injury to intestines as well as livers, kidneys, and lungs, evidenced by morphological alteration, increased cell apoptosis, and elevated serum levels of ALT, AST, BUN, and Cr. The damage reached peak 3 h after II/R. The intestinal LPO and serum levels of TNF-α were increased after II/R. Pre-administration of taurine significantly attenuated multiple organ injury as the histological score, apoptosis index, LPO, and levels of ALT, AST, BUN, Cr, and TNF-α were significantly lower compared with saline controls.
Conclusions
Taurine attenuates multiple organ injury induced by II/R. Although the mechanism needs further investigation, taurine inhibits production of intestinal LPO, release of TNF-α, cell apoptosis, and expression of caspase-3.