Identification of novel HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from Zinc Transporter 8

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• 作者：Shufa Li^a ; ^b ; ^{shufali@163.com} ; Haiying Li^b ; Bing Chen^c ; Debin Lu^c ; Wuquan Deng^c ; Youzhao Jiang^c ; Zhongqi Zhou^d ; Zhao Yang^a ; ^{yangzhao5140@sohu.com}
• 关键词：HLA-A*0201 ; Cytotoxic T lymphocyte ; Epitopes ; Zinc Transporter 8
• 刊名：Vaccine
• 出版年：2013
• 出版时间：15 March, 2013
• 年：2013
• 卷：31
• 期：12
• 页码：1610-1615
• 全文大小：770 K

文摘

Numerous evidences demonstrated that type 1 diabetes (T1D) is due to a loss of immune tolerance to islet antigens, and CD8⁺ T cells play an important role in the development of T1D. Zinc Transporter 8 (ZnT8) has emerged in recent years as a target of disease-associated autoreactive T cells in human T1D. However, ZnT8-associated CTL specific-peptides have not been identified. In this study, we predicted and identified HLA-A*0201-restricted cytotoxic T lymphocyte (CTL) epitopes derived from ZnT8, and utilized it to immunize HLA-A2.1/Kb transgenic (Tg) mice. The results demonstrated that peptides of ZnT8 containing residues 107-115, 115-123 and 145-153 could elicit specific CTLs in vitro, and induce diabetes in mice. The results suggest that these specific peptides are novel HLA-A*0201-restricted CTL epitopes, and could have therapeutic potential in preventing of T1D disease.