High-performance thin-layer chromatography as a fast screening tool for phosphorylated peptides
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- 作者：Lena Morschheusera ; lena.morschheuser@chemie.uni-hamburg.de" class="auth_mail" title="E-mail the corresponding author ; Sandra Mü ; kuschb ; c ; sandra.muekusch@izi-bb.fraunhofer.de" class="auth_mail" title="E-mail the corresponding author ; Maria Riednerd ; maria.riedner@chemie.uni-hamburg.de" class="auth_mail" title="E-mail the corresponding author ; Harald Seitzb ; harald.seitz@izi-bb.fraunhofer.de" class="auth_mail" title="E-mail the corresponding author ; Sascha Rohna ; rohn@chemie.uni-hamburg.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：High-performance thin-layer chromatography ; Phosphopeptide detection ; Nociceptive pathway ; HPTLC-MALDI-TOF-MS ; HPTLC-immunostaining
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：1008
- 期：Complete
- 页码：198-205
- 全文大小：1079 K
文摘
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An immunological detection of phosphopeptides after HPTLC was developed.
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Phosphopeptides were detected applying monoclonal antibodies.
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Via HPTLC-MALDI-TOF a simultaneous characterization of the peptides was realized.