Prediction of in vivo developmental toxicity by combination of Hand1-Luc embryonic stem cell test and metabolic stability test with clarification of metabolically inapplicable candidates
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- 作者：Hirohisa Nagahoria ; nagahori@sc.sumitomo-chem.co.jp" class="auth_mail" title="E-mail the corresponding author ; Noriyuki Suzukia ; Florian Le Coza ; Takashi Omorib ; Koichi Saitoa
- 关键词：EST ; embryonic stem cell test ; ECVAM ; European Center for the Validation of Alternative Methods ; PBPK ; physiologically based pharmacokinetics ; MIC ; metabolically inapplicable candidate ; PBS ; phosphate buffered saline ; DMSO ; dimethyl sulfoxide ; MD ; maximum dose ; ESI ; electrospray ionization ; Rt ; retention time ; Fh ; hepatic availability ; ROC ; receiver operating characteristic curve ; PM ; prediction model ; IC50 ; the concentration that reduces the viability of cells to 50% ; ID50 ; the concentration th
- 刊名：Toxicology Letters
- 出版年：2016
- 出版时间：30 September 2016
- 年：2016
- 卷：259
- 期：Complete
- 页码：44-51
- 全文大小：961 K
文摘
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A step-wise prediction with Hand1-Luc EST and metabolic stability test is proposed.
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Hand1-Luc EST of a total of 71 chemicals shows highly reproducible results.
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MICs potentially have a metabolic activation and accumulation properties.
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Exclusion of MICs significantly improves classification of developmental toxicity.