Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis
Mixed mode LC fractionation coupled with reversed phase LC-MS/MS for lipidomics. 22 lipid classes were separated in mixed mode on a monolithic Si-based column. Reduced ion suppression and cleaner spectra obtained from fractionated samples. Total fatty acid analysis to improve confidence of identified lipid in LipidSearch. More than 800 lipids were confidentially identified in rat plasma and rat liver.