Restoration of arsenite induced hepato-toxicity by crude tannin rich fraction of Theobroma cacao in Sprague Dawley rats

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• 作者：Chandronitha Chandranayagam^a ; Gayathri Veeraraghavan^a ; Ananthi Subash^a ; Hannah Rachel Vasanthi^a ; ^b ; ^{hrvasanthi@gmail.com}
• 关键词：GSH ; reduced glutathione ; GPx ; glutathione peroxidase ; SOD ; superoxide dismutase ; NOx ; nitrite/nitrate ; ALAD ; delta-aminolevulinic acid dehydratase ; TBARS ; thiobarbituric acid reactive substances ; TCF ; tannin rich cocoa fraction ; DCF ; detannified cocoa fr
• 刊名：Food Research International
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：50
• 期：1
• 页码：46-54
• 全文大小：1196 K

文摘

The present study was designed to investigate the protective efficacy of tannin rich cocoa (Theobroma cacao L.) in comparison with its detannified fraction. Arsenic as sodium-m-arsenite at 100 ppm dose was orally administered via drinking water constantly for 28 days to Sprague Dawley female rats. The hepatic cellular thiol levels such as total sulfhydryl content, protein bound sulfhydryl, non-protein bound sulfhydryl content, cellular oxidative stress and damage markers such as reduced glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD) and the index of nitrite/nitrate (NO_x) levels and a biomarker for arsenic toxicity namely delta-aminolevulinic acid dehydratase (ALAD) were substantially reduced and elevation of thiobarbituric acid reactive substances (TBARS) was observed in arsenic-exposed groups. The supplementation of tannin rich cocoa fraction (TCF) enhanced the levels of cellular thiols, markers of oxidative stress and levels of arsenic biomarkers and decreased the level of oxidative damage in treatment groups. Histopathologically, the liver of arsenic-exposed rats showed some degenerative lesions with few cells showing appearance of apoptosis which was effectively antagonized by tannin rich cocoa feeding. Treatment with detannified cocoa (DCF) was not that protective compared to the tannin rich cocoa (TCF). These results demonstrate that tannin rich cocoa can be considered as a functional food which effectively antagonizes many of the adverse effects of arsenic intoxication.