Fed-batch production and secretion of streptavidin by Hansenula polymorpha: Evaluation of genetic factors and bioprocess development
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- 作者：David Wetzel1 ; Jakob M. Mü ; ller1 ; jmu@fermtech.techfak.uni-bielefeld.de" class="auth_mail" title="E-mail the corresponding author ; Erwin Flaschel ; Karl Friehs ; Joe M. Risse
- 关键词：α-MF ; α-mating factor ; SAV ; streptavidin ; vvm ; volume per volume and minute
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 May 2016
- 年：2016
- 卷：225
- 期：Complete
- 页码：3-9
- 全文大小：978 K
文摘
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Streptavidin (SAV) production by the yeast Hansenula polymorpha was established.
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Two strains, FMD and MOX promoter, and various SAV genes were analyzed.
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Cultivation at 30 °C and FMD-driven expression of full-length SAV was beneficial.
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Methanol-free, derepression-based expression led to 1.3 μM of SAV in 48 h.
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Induction by methanol yielded 11.42 μM of highly bioactive SAV in 216 h.