A method for the preparation of curcumin by ultrasonic-assisted ammonium sulfate/ethanol aqueous two phase extraction

详细信息    查看全文

• 作者：Guangkuan Xu ; Changchun Hao ; Suyang Tian ; Feng Gao ; Wenyuan Sun ; Runguang Sun ; ^{Biophymed@snnu.edu.cn}
• 关键词：Curcumin ; Aqueous two-phase system ; Ultrasonic-assisted ; Response surface methodology ; Semi-preparative HPLC
• 刊名：Journal of Chromatography B
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：1041-1042
• 期：Complete
• 页码：167-174
• 全文大小：1571 K
• 卷排序：1041

文摘

This study investigated a new and easy-to-industrialized extracting method for curcumin from Curcuma longa rhizomes using ultrasonic extraction technology combined with ammonium sulfate/ethanol aqueous two-phase system (ATPS), and the preparation of curcumin using the semi-preparative HPLC. The single-factor experiments and response surface methodology (RSM) were utilized to determine the optimal material-solvent ratio, ultrasonic intensity (UI) and ultrasonic time. The optimum extraction conditions were finally determined to be material-solvent rate of 3.29:100, ultrasonic intensity of 33.63 W/cm2 and ultrasonic time of 17 min. At these optimum conditions, the extraction yield could reach 46.91 mg/g. And the extraction yields of curcumin remained stable in the case of amplification, which indicated that scale-up extraction was feasible and efficient. Afterwards, the semi-preparative HPLC experiment was carried out, in which optimal preparation conditions were elected according to the single factor experiment. The prepared curcumin was obtained and the purity could up to 85.58% by the semi-preparative HPLC.