Low-potential determination of hydrogen peroxide, uric acid and uricase based on highly selective oxidation of p-hydroxyphenylboronic acid by hydrogen peroxide

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• 作者：Feng Liang^a ; ^bAuthor Vitae ; Lianzhe Hu^a ; ^cAuthor Vitae ; Yunhui Li^bAuthor Vitae ; Saadat Majeed^a ; ^dAuthor Vitae ; Haidong Li^aAuthor Vitae ; Haoran Cai^aAuthor Vitae ; Xiuyun Yang^b ; ^{yangxiuyun1@126.com}Author Vitae ; Guobao Xu^a ; ^{guobaoxu@ciac.jl.cn}Author Vitae
• 关键词：Electrochemistry ; Hydroxyphenol ; Hydrogen peroxide ; Uric acid ; Uricase
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：1 March, 2013
• 年：2013
• 卷：178
• 期：Complete
• 页码：144-148
• 全文大小：782 K

文摘

A new strategy for the electrochemical determination of hydrogen peroxide (H₂O₂), uric acid (UA), and urate oxidase (UOx) has been developed. The strategy is based on the indirect electrochemical detection of hydrogen peroxide through detecting p-hydroxyphenol generated from the selective oxidation of p-hydroxyphenylboronic acid by hydrogen peroxide. As it is easy to detect p-hydroxyphenol with excellent selectivity at low potential, the sensors display satisfactory analytical performance, such as wide dynamic range, high sensitivity, and good selectivity. The sensor exhibits a linear response over the range of 1 ¦ÌM to 1 mM, 1-500 ¦ÌM, and 0.005-0.1 U/mL with the detection limits of 0.31 ¦ÌM, 0.3 ¦ÌM, and 0.005 U/mL for hydrogen peroxide, UA, and UOx, respectively. Furthermore, the sensor exhibited excellent recoveries for the determination of UA in serum samples. The detection scheme is very simple, and can be readily extended to detect other oxidases and their substrates.