Allantoin in human urine quantified by ultra-performance liquid chromatography–tandem mass spectrometry

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• 作者：Adviye A. Tolun ; Haoyue Zhang ; Dora Il’ ; yasova ; Judit Sztá ; ray ; Sarah P. Young ; David S. Millington
• 刊名：Analytical Biochemistry
• 出版年：2010
• 出版时间：15 July 2010
• 年：2010
• 卷：402
• 期：2
• 页码：191-193
• 全文大小：214 K

文摘

Uric acid is a potent antioxidant and scavenger of singlet oxygen and other radicals in humans. Allantoin, the predominant product of free radical-induced oxidation of uric acid, is efficiently excreted in the urine and has potential as a biomarker of oxidative stress. We developed a rapid and specific assay for urinary allantoin using ultra-performance liquid chromatography–tandem mass spectrometry suitable for high-throughput clinical studies. The method required minimal sample preparation and was accurate (mean error = 6 % ), precise (intra- and interday imprecision <8 % ), and sensitive (limit of detection = 0.06 pmol). Allantoin levels measured in control samples were comparable to literature values.