文摘
F2-isoprostanes are useful biomarkers of oxidative status in humans. We developed an ultraperformance liquid chromatography–tandem mass spectrometric (UPLC–MS/MS) method to quantify 2,3-dinor-8-iso prostaglandin F2α, a urinary metabolite of 8-iso-prostaglandin F2α. Urine was purified by solid-phase extraction and analyzed by UPLC–MS/MS with negative-ion electrospray ionization. The method was robust with a mean inaccuracy of 9 % , interday and intraday imprecision of 7.5 % or lower, and a lower limit of quantification of 0.5 μg/L, equivalent to 0.04 pmol injected onto the column. An analysis time of 6 min was shorter than previously published methods and amenable to large studies.