Quantification of the oxidative damage biomarker 2,3-dinor-8-isoprostaglandin-F_2α in human urine using liquid chromatography–tandem mass spectrometry

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• 作者：Haoyue Zhang ; Dora Il’ ; yasova ; Judit Sztaray ; Sarah P. Young ; Frances Wang ; David S. Millington
• 刊名：Analytical Biochemistry
• 出版年：2010
• 出版时间：15 April 2010
• 年：2010
• 卷：399
• 期：2
• 页码：302-304
• 全文大小：245 K

文摘

F₂-isoprostanes are useful biomarkers of oxidative status in humans. We developed an ultraperformance liquid chromatography–tandem mass spectrometric (UPLC–MS/MS) method to quantify 2,3-dinor-8-iso prostaglandin F_2α, a urinary metabolite of 8-iso-prostaglandin F_2α. Urine was purified by solid-phase extraction and analyzed by UPLC–MS/MS with negative-ion electrospray ionization. The method was robust with a mean inaccuracy of 9 % , interday and intraday imprecision of 7.5 % or lower, and a lower limit of quantification of 0.5 μg/L, equivalent to 0.04 pmol injected onto the column. An analysis time of 6 min was shorter than previously published methods and amenable to large studies.