- 作者：Toko Harata ; Hisao Ando ; Akira Iwase ; Tetsuro Nagasaka ; Shigehiko Mizutani ; Fumitaka Kikkawa
- 关键词：Adipocyte-derived leucine aminopeptidase (ER-aminopeptidase 1) ; aminopeptidase A ; angiotensin converting enzyme ; angiotensin II ; AT1 receptor ; corpus luteum ; immunohistochemistry ; ovary ; vascular endothelial growth factor
- 刊名：Fertility and Sterility
- 出版年：2006
- 出版时间：August 2006
- 年：2006
- 卷：86
- 期：2
- 页码：433-439
- 全文大小：667 K
Objective
To assess the expression and cellular distribution of angiotensin II (Ang II), angiotensin type 1 receptor (AT1R), angiotensin-converting enzyme (ACE), aminopeptidase A (APA), adipocyte-derived leucine aminopeptidase (A-LAP), and vascular endothelial growth factor (VEGF) in human ovarian tissue during the menstrual cycle.Design
Ovarian tissues (n = 52) and corpora lutea (n = 34) were obtained from patients undergoing hysterectomy/oophorectomy, and tissue sections were immunostained for each antigen.
Setting
University hospital.
Patient(s)
Patients undergoing hysterectomy or oophorectomy for benign conditions.
Intervention(s)
Immunostaining of tissue sections using antibodies to each antigen.
Main Outcome Measure(s)
Microscopic evaluation to assess the presence, distribution, and cellular localization.
Result(s)
The luteal tissue is the major site of Ang II, ACE, AT1R, and VEGF, with highest staining intensity found during the midluteal phase and at pregnancy. The AT1R was found in theca cells. The APA was strongly immunolocalized in pericytes. Immunolocalization of AT1R was almost similar to that of VEGF including oocytes in the primordial and intermediate follicles.
Conclusion(s)
The expression and distinct pattern of the cellular localization of Ang II and its related proteins in human ovarian tissue during folliculogenesis and in the luteal tissue suggest their roles in the growth and differentiation of theca, granulose, and luteal cells.