ESI-FTICR mass spectrometry employing data-dependent external ion selection and accumulation

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• 作者：Harkewicz ; Richard ; Belov ; Mikhail E. ; Anderson ; Gordon A. ; Pa&scaron ; a-Tolic ; Ljiljana ; et. al.
• 刊名：Journal of the American Society for Mass Spectrometry
• 出版年：2001
• 出版时间：February, 2001
• 年：2001
• 卷：13
• 期：2
• 页码：144-154
• 全文大小：281 K

文摘

Data-dependent external m/z selection and accumulation of ions is demonstrated in use with ESI-FTICR instrumentation, with two different methods for ion selection being explored. One method uses RF/DC quadrupole filtering and is described in use with an 11.5 tesla (T) FTICR instrument, while the second method employs RF-only resonance dipolar excitation selection and is described in use with a 3.5 T FTICR instrument. In both methods ions are data-dependently selected on the fly in a linear quadrupole ion guide, then accumulated in a second linear RF-only quadrupole trap that immediately follows. A major benefit of ion preselection prior to external accumulation is the enhancement of ion populations for low-level species. This development is expected to expand the dynamic range and sensitivity of FTICR for applications including analysis of complex polypeptide mixtures (e.g., proteomics).