High-throughput lipidomic analysis of fatty acid derived eicosanoids and N-acylethanolamines

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• 作者：Darren S.  ; Dumlao¹ ; Matthew W.  ; Buczynski¹ ; Paul C.  ; Norris ; Richard  ; Harkewicz ; Edward A.  ; Dennis ;   ; ^{edennis@ucsd.edu}
• 关键词：Eicosanoid ; N-acylethanolamine ; Lipidomics ; LIPID MAPS ; High-throughput methodology ; Mass spectrometry
• 刊名：Biochimica et Biophysica Acta (BBA)/Molecular and Cell Biology of Lipids
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：1811
• 期：11
• 页码：724-736
• 全文大小：1323 K

文摘

Fatty acid-derived eicosanoids and N-acylethanolamines (NAE) are important bioactive lipid mediators involved in numerous biological processes including cell signaling and disease progression. To facilitate research on these lipid mediators, we have developed a targeted high-throughput mass spectrometric based methodology to monitor and quantitate both eicosanoids and NAEs, and can be analyzed separately or together in series. Each methodology utilizes scheduled multiple reaction monitoring (sMRM) pairs in conjunction with a 25 min reverse-phase HPLC separation. The eicosanoid methodology monitors 141 unique metabolites and quantitative amounts can be determined for over 100 of these metabolites against standards. The analysis covers eicosanoids generated from cycloxygenase, lipoxygenase, cytochrome P450 enzymes, and those generated from non-enzymatic pathways. The NAE analysis monitors 36 metabolites and quantitative amounts can be determined for 33 of these metabolites against standards. The NAE method contains metabolites derived from saturated fatty acids, unsaturated fatty acids, and eicosanoids. The lower limit of detection for eicosanoids ranges from 0.1 pg to 1 pg, while NAEs ranges from 0.1 pg to 1000 pg. The rationale and design of the methodology is discussed. This article is part of a Special Issue entitled Lipodomics and Imaging Mass Spectrometry.