IGF-1 prevents oxidative stress induced-apoptosis in induced pluripotent stem cells which is mediated by microRNA-1

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• 作者：Yangxin Li ; Harnath Shelat ; Yong-Jian Geng
• 关键词：iPS cells ; IGF-1 ; microRNA ; Apoptosis ; Cytochrome-c ; Mitochondria
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2012
• 出版时间：5 October, 2012
• 年：2012
• 卷：426
• 期：4
• 页码：615-619
• 全文大小：574 K

文摘

Oxidative stress contributes to tissue injury and cell death during the development of various diseases. The present study aims at investigating whether oxidative stress triggered by the exposure to hydrogen peroxide (H₂O₂) can induce apoptosis of induced pluripotent stem cells (iPS cells) in a mechanism mediated by insulin-like growth factor (IGF-1) and microRNA-1 (miR-1). iPS cells treated with H₂O₂ showed increases in miR-1 expression, mitochondria dysfunction, cytochrome-c release and apoptosis, Addition of IGF-1 into the iPS cell cultures reduced the H₂O₂ cytotoxicity. Prediction algorithms showed that 3¡ä-untranslated regions of IGF-1 gene as a target of miR-1. Moreover, miR-1 mimic, but not miR-1 mimic negative control, diminished the protective effect of IGF-1 on H₂O₂-induced mitochondrial dysfunction, cytochrome-c release and apoptosis in iPS cells. In conclusion, IGF-1 inhibits H₂O₂-induced mitochondrial dysfunction, cytochrome-c release and apoptosis. IGF-1¡äs effect is, at least partially, regulated by miR-1 in iPS cells.