A Genetically Encoded Probe for Live-Cell Imaging of H4K20 Monomethylation
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- 作者：Yuko Sato1 ; satoy@bio.titech.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Tomoya Kujirai2 ; Ritsuko Arai3 ; 9 ; Haruhiko Asakawa4 ; Chizuru Ohtsuki4 ; Naoki Horikoshi2 ; Kazuo Yamagata5 ; Jun Ueda6 ; Takahiro Nagase7 ; Tokuko Haraguchi4 ; 8 ; Yasushi Hiraoka4 ; 8 ; Akatsuki Kimura3 ; Hitoshi Kurumizaka2 ; Hiroshi Kimura1 ; hkimura@bio.titech.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：H4K20me1 ; histone H4 Lys20 monomethylation ; Mintbody ; modification-specific intracellular antibody ; scFv ; single-chain variable fragment ; PCNA ; proliferating cell nuclear antigen ; FR ; framework region ; Xi ; inactive X chromosome
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：9 October 2016
- 年：2016
- 卷：428
- 期：20
- 页码：3885-3902
- 全文大小：1853 K
文摘
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A histone H4K20me1-specific live-cell probe, H4K20me1-mintbody, was developed.
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Dynamics and replication timing of Xi were visualized.
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Critical amino acids for the stability and/or folding of the mintbody were revealed.