Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport

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• 作者：Yoshinori Makino ; Tadaki Suzuki ; Rie Hasebe ; Takashi Kimura ; Akihiko Maeda ; Hidehiro Takahashi ; Hirofumi Sawa
• 关键词：West Nile virus ; Microtubules ; Viral entry
• 刊名：Journal of Virological Methods
• 出版年：January, 2014
• 年：2014
• 卷：195
• 期：Complete
• 页码：250-257
• 全文大小：1928 K

文摘

West Nile virus (WNV) is one of flaviviruses and has emerged recently in the United States as a significant cause of viral encephalitis. Although cellular entry of WNV is important for viral pathogenesis, its mechanisms have not been elucidated fully. To explore the entry mechanisms, a virus-particle tracking system in live cells by using fluorescently labeled subviral particles (SVPs) and time-lapse epifluorescence microscopy was established. This study revealed that, following cellular entry, SVP movements could be divided into two phases: early (slow movement) and late (fast movement) phase. Moreover, fast viral particle movements at the late phase correlated with SVP-microtubule association.