文摘
Sofosbuvir metabolite was studied for the first time by Raman spectroscopy. Cheap and disposable paper substrate was utilized for the determination of PSI-6206 by HPLC-SERS. The use of disposable substrate eliminated the memory effect problem in the HPLC-SERS. The SERS LOQ of PSI-6206 was 13 ng L-1 (R2 = 0.959, RSD = 5.23%), 4 orders of magnitude less than HPLC-DAD. The HPLC-SERS method provided unique structural identification of PSI-6206 similar to HPLC-MS