Preparation, evaluation and application of diazinon imprinted polymers as the sorbent in molecularly imprinted solid-phase extraction and liquid chromatography analysis in cucumber and aqueous samples

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• 作者：Davood Davoodi^a ; Mohammad Hassanzadeh-Khayyat^b ; Mitra Asgharian Rezaei^b ; Seyed Ahmad Mohajeri^b ; ^{mohajeria@mums.ac.ir" class="auth_mail}
• 关键词：AIBN ; 2 ; 2&prime ; -azo-bis-iso-butyronitrile ; Bmax ; maximum binding sites ; EGDMA ; ethylene glycol dimethacrylate ; KD ; dissociation constant ; MAA ; methacrylic acid ; MISPE ; molecularly imprinted solid-phase extraction ; NIP ; non-imprinted polymer ; ACN ; acetonitrile
• 刊名：Food Chemistry
• 出版年：1 September, 2014
• 年：2014
• 卷：158
• 期：Complete
• 页码：421-428
• 全文大小：686 K

文摘

A series of diazinon imprinted polymers (MIPs) were prepared and evaluated in the binding study in comparison with a non-imprinted polymer (NIP). The optimised MIP was evaluated as a sorbent, for extraction and preconcentration of diazinon from aqueous media and cucumber tissue. The HPLC-UV method was calibrated, in the range of 0.025-10 mg/kg. The results indicated that the optimised MIP had an excellent affinity for diazinon. The molecularly imprinted solid-phase extraction (MISPE) procedure was optimised with a recovery of 77-98%, in aqueous solution, and a recovery of 82-110%, in cucumber. The intra-day variation and inter-day variation values were less than 8.26% and 9.7%, respectively. Our data showed that, the MIP enabled the extraction of trace amounts of diazinon successfully from aqueous solution and cucumber, demonstrating the potential of MISPE for rapid, sensitive and cost-effective sample analysis.