Synthetic assembly of novel avidin-biotin-GlcNAc (ABG) complex as an attractive bio-probe and its interaction with wheat germ agglutinin (WGA)
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- 作者：Amrita Kumari ; Tetsuo Koyama ; Ken Hatano ; Koji Matsuoka ; koji@fms.saitama-u.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bio-probe ; Biotin ; Lectin ; Glycocluster ; Tryptophan ; Multivalency
- 刊名：Bioorganic Chemistry
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：68
- 期：Complete
- 页码：219-225
- 全文大小：855 K
文摘
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GlcNAc, biotin, and avidin were used for this study.
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GlcNAc derivatization gave GlcNAc-biotin conjugate.
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Tetrameric avidin-biotin-GlcNAc (ABG complex) was accomplished by known avidin-biotin interaction.
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Fluorometric assay of the ABG complex against WGA lectin gave high association constant due to the glycocluster effect.