A novel yeast cell-based screen identifies flavone as a tankyrase inhibitor

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• 作者：Yoko Yashiroda ; Reika Okamoto ; Kaori Hatsugai ; Yasushi Takemoto ; Naoki Goshima ; Tamio Saito ; Makiko Hamamoto ; Yoshikazu Sugimoto ; Hiroyuki Osada ; Hiroyuki Seimiya ; Minoru Yoshida
• 关键词：Tankyrase 1 ; Inhibitor ; High-throughput screening ; Schizosaccharomyces pombe
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：9 April 2010
• 年：2010
• 卷：394
• 期：3
• 页码：569-573
• 全文大小：459 K

文摘

The telomere-associated protein tankyrase 1 is a poly(ADP-ribose) polymerase and is considered to be a promising target for cancer therapy, especially for BRCA-associated cancers. However, an efficient assay system for inhibitor screening has not been established, mainly due to the difficulty of efficient preparation of the enzyme and its substrate. Here, we report a cell-based assay system for detecting inhibitory activity against tankyrase 1. We found that overexpression of the human tankyrase 1 gene causes a growth defect in the fission yeast Schizosaccharomyces pombe. Chemicals that restore the growth defect phenotype can be identified as potential tankyrase 1 inhibitors. We performed a high-throughput screen using this system, and identified flavone as a compound that restores the growth of yeast cells overexpressing tankyrase 1. Indeed, flavone inhibited poly(ADP-ribosyl)ation of proteins caused by overexpression of tankyrase 1 in yeast cells. This system allows rapid identification of inhibitory activity against tankyrase 1 and is amenable to high-throughput screening using robotics.