Methods for investigation of targeted kinase inhibitor therapy using chemical proteomics and phosphorylation profiling
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- 作者：Bin Fang ; Eric B. Haura ; Keiran S. Smalley ; Steven A. Eschrich ; John M. Koomen
- 关键词：Phosphorylation ; Phosphoproteomics ; Mass spectrometry ; Drug response ; Drug resistance ; Targeted therapy
- 刊名：Biochemical Pharmacology
- 出版年：2010
- 出版时间：1 September 2010
- 年：2010
- 卷：80
- 期：5
- 页码：739-747
- 全文大小：451 K
文摘
Phosphorylation acts as a molecular switch for many regulatory events in signaling pathways that drive cell division, proliferation, and apoptosis. Because of the critical nature of these protein post-translational modifications in cancer, drug development programs often focus on inhibitors for kinases and phosphatases, which control protein phosphorylation. Numerous kinase inhibitors have entered clinical use, but prediction of their efficacy and a molecular basis for patient response remain uncertain. Chemical proteomics, the combination of drug affinity chromatography with mass spectrometry, identifies potential target proteins that bind to the drugs. Phosphorylation profiling can complement chemical proteomics by cataloging modifications in the target kinases and their downstream substrates using phosphopeptide enrichment and quantitative mass spectrometry. These experiments shed light on the mechanism of disease development and illuminate candidate biomarkers to guide personalized therapeutic strategies. In this review, commonly applied technologies and workflows are discussed to illustrate the role of proteomics in examining tumor biology and therapeutic intervention using kinase inhibitors.