A novel primer set for improved direct gene sequencing of human bocavirus genotype-1 from clinical samples
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- 作者：Ahmed S. Abdel-Moneima ; b ; asa@tu.edu.sa" class="auth_mail" title="E-mail the corresponding author ; asa@bsu.edu.eg" class="auth_mail" title="E-mail the corresponding author ; Mahmoud M. Kamelc ; Dina H. Hamedd ; Safaa S. Hassanc ; May S. Solimane ; Saleh A. Al-Quraishyf ; Amani A. El Kholye
- 关键词：Human bocavirus ; Respiratory tract infection in children ; Genotyping ; Nosocomial infection ; Egypt
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：228
- 期：Complete
- 页码：108-113
- 全文大小：997 K
文摘
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HBoV is a parvovirus that causes respiratory and enteric diseases in humans.
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A new primer set used in conventional PCR and direct gene sequencing was developed.
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The new primer set detected all real-time positive samples.
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Genotying of HBoV strains was conducted for the first time in Egypt.
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High rate of nosocomial infection with HBoV-1 was detected in children in Egypt.