In vivo expression of human cytomegalovirus (HCMV) microRNAs during latency
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- 作者：Mesfin K. Mesheshaa ; Zvi Bentwicha ; Semaria A. Solomona ; Yonat Shemer Avnia ; b ; yonat@bgu.ac.il" class="auth_mail" title="E-mail the corresponding author
- 关键词：cDNA ; DNA complementary to RNA ; dsDNA ; double stranded DNA ; EBV ; Epstein&ndash ; Barr virus ; GB ; major envelop glycoprotein gene ; HCMV ; human cytomegalovirus ; HFF ; human foreskin fibroblast cells ; HIV ; human immunodeficiency virus ; IE ; immediate early ; Ig ; immunoglobulin ; LUNA ; latency unique nuclear antigen ; MIEP ; major immediate early promotor ; miRNAs ; microRNAs ; MOI ; multiplicity of infection ; mRNA ; messenger RNA ; PBMC ; peripheral mononuclear cells ; PBS ; phosphate buffer saline ; PCR ; polymerase
- 刊名：Gene
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：575
- 期：1
- 页码：101-107
- 全文大小：875 K
文摘
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HCMV micrRNAs expressed during latency
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Distinct expression of only 9 HCMV-miRs during CMV latency
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Reactivation from latency restores expression of all HCMV encoded microRNAs.
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HCMV alternatively expresses the two arms of mir-US29 in lytic and latency phases.
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Application of the approach useful to study other viral miRNAs during latency