文摘
Affibody molecules are small (58 amino acids) high-affinity proteins based on a tri-helix non-immunoglobulin scaffold. A clinical study has demonstrated that PET imaging using Affibody molecules labeled with 68Ga (T½xA0;=xA0;68xA0;min) can visualize metastases of breast cancer expressing human epidermal growth factor receptor type 2 (HER2) and provide discrimination between tumors with high and low expression level. This may help to identify breast cancer patients benefiting from HER2-targeting therapies. The best discrimination was at 4xA0;h post injection. Due to longer half-life, a positron-emitting radionuclide 44Sc (T½xA0;=xA0;4.04xA0;h) might be a preferable label for Affibody molecules for imaging at several hours after injection.MethodsA synthetic second-generation anti-HER2 Affibody molecule ZHER2:2891 was labeled with 44Sc via a DOTA-chelator conjugated to the N-terminal amino group. Binding specificity, affinity and cellular processing 44Sc-DOTA-ZHER2:2891 and 68Ga-DOTA-ZHER2:2891 were compared in vitro using HER2-expressing cells. Biodistribution and imaging properties of 44Sc-DOTA-ZHER2:2891 and 68Ga-DOTA-ZHER2:2891 were evaluated in Balb/c nude mice bearing HER2-expression xenografts.ResultsThe labeling yield of 98xA0;±xA0;2% and specific activity of 7.8xA0;GBq/μmol were obtained. The conjugate demonstrated specific binding to HER2-expressing SKOV3.ip cells in vitro and to SKOV3.ip xenografts in nude mice. The distribution of radioactivity at 3xA0;h post injection was similar for 44Sc-DOTA-ZHER2:2891 and 68Ga-DOTA-ZHER2:2891, but the blood clearance of the 44Sc-labeled variant was slower and the tumor-to-blood ratio was reduced (15xA0;±xA0;2 for 44Sc-DOTA-ZHER2:2891 vs 46xA0;±xA0;9 for 68Ga-DOTA-ZHER2:2891). At 6xA0;h after injection of 44Sc-DOTA-ZHER2:2891 the tumor uptake was 8xA0;±xA0;2% IA/g and the tumor-to-blood ratio was 51xA0;±xA0;8. Imaging using small-animal PET/CT demonstrated that 44Sc-DOTA-ZHER2:2891 provides specific and high-contrast imaging of HER2-expressing xenografts.ConclusionThe 44Sc- DOTA-ZHER2:2891 Affibody molecule is a promising probe for imaging of HER2-expression in malignant tumors.