文摘
An LC–NSI–HRMS/MS method was developed to quantify NNN enantiomers in human urine. [Pyridine-D4]nornicotine was used to monitor artifactual formation of NNN. (S)-NNN, the more carcinogenic enantiomer, predominated in human urine. The enantiomeric ratio in urine was remarkably similar to that in tobacco products. This is the first use of NSI-HRMS for chiral separation of urinary metabolites.