Chromatographic evaluation of structure selective and enantioselective retention of amines and acids on cellobiohydrolase I wild type and its mutant D214N

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• 作者：Hedeland ; Mikael ; et. al.
• 关键词：Enantiomer separation ; Chiral stationary phases ; LC ; Cellobiohydrolase stationary phases ; Amines ; Organic acids ; Amino alcohols ; Warfarin ; Ditoluoyltartaric acid ; Dibenzoyltartaric acid
• 刊名：Journal of Chromatography A
• 出版年：1999
• 出版时间：December 9, 1999
• 年：1999
• 卷：864
• 期：1
• 页码：1-16
• 全文大小：266 K

文摘

The mechanisms of structure selective and enantioselective retentions of amines and acids on two chiral stationary phases based on wild type cellobiohydrolase I (CBH I) and its mutant D214N have been investigated. All the amino alcohols tested had an enantioselective site that overlaps with the catalytically active site of CBH I, whereas the enantioselectivity of prilocaine was not affected by the mutation. The hydroxyl group of the amino alcohols did not seem to be an important contributor to the total binding strength whereas a bromo substituent in the aromatic ring promotes a high enantioselectivity (α