Non-aqueous capillary electrophoretic separation of enantiomeric amines with (−)-2,3:4,6-di-O-isopropylidene-2-keto-l-gulonic acid as chiral counter ion

详细信息    查看全文

• 作者：Carlsson ; Ylva ; Hedeland ; Mikael ; Bondesson ; Ulf ; Pettersson ; Curt
• 关键词：Non-aqueous capillary electrophoresis ; Enantiomer separation ; Background electrolyte composition ; Amines ; Diisopropylideneketogulonic acid ; Labetalol
• 刊名：Journal of Chromatography A
• 出版年：2001
• 出版时间：July 13, 2001
• 年：2001
• 卷：922
• 期：1-2
• 页码：303-311
• 全文大小：185 K

文摘

(−)-2,3:4,6-Di-O-isopropylidene-2-keto-l-gulonic acid [(−)-DIKGA] has been introduced as a chiral counter ion in non-aqueous capillary electrophoresis. High enantioresolutions (R_S≥3) were obtained for amines, e.g., pronethalol, labetalol and bambuterol. Methanol containing NaOH and (−)-DIKGA was used as the background electrolyte. The counter ion concentration and the nature of the injection medium were found to affect the chiral separation. Covalent coating of the fused-silica capillary reduced the electro-osmotic flow resulting in improved enantioresolutions.