Shedding of procoagulant microparticles from unstimulated platelets by integrin-mediated destabilization of actin cytoskeleton

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• 作者：Sandra Cauwenberghs ; Marion A.H. Feijge ; Alan G.S. Harper ; Stewart O. Sage ; Joyce Curvers and Johan W.M. Heemskerk
• 关键词：Actin cytoskeleton ; Calpain ; Integrin α ; IIbβ ; 3 ; Microparticles ; Procoagulant activity
• 刊名：FEBS Letters
• 出版年：2006
• 出版时间：2 October 2006
• 年：2006
• 卷：580
• 期：22
• 页码：5313-5320
• 全文大小：270 K

文摘

Platelet activation by potent, Ca²⁺-mobilizing agonists results in shedding of microparticles that are active in coagulation. Here we show that platelets under storage produce procoagulant microparticles in the absence of agonist. Microparticle formation by resting platelets results from αIIbβ3 signaling to destabilization of the actin cytoskeleton in the absence of calpain activation. Integrin-mediated spreading of platelets over fibrinogen similarly results in microparticle formation. After transfusion of stored platelet preparations to thrombocytopenic patients, the microparticles contribute to coagulant activity in vivo.