Increased thermal stability of cold-adapted esterase at ambient temperatures by immobilization on graphene oxide

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• 作者：Heeyoung Lee ; Hae Kyung Jeong ; Jinwoo Han ; Hye-Shin Chung ; Sei-Heon Jang ; ChangWoo Lee
• 关键词：Graphene oxide ; Cold-adapted enzyme ; Immobilization ; Thermal stability
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：148
• 期：Complete
• 页码：620-623
• 全文大小：429 K

文摘

In this study, the effect of graphene oxide (GO) on the thermal stability of a recombinant esterase from cold-adapted Pseudomonas mandelii, rEstKp, was investigated. The complex GO-rEstKp was formed by cross-linking. Both free rEstKp and GO-rEstKp complex showed similar optimum pH and temperatures. GO-rEstKp complex exhibited enhanced thermal stability at ambient temperatures than rEstKp, which prevented the denaturation of the enzyme by hydrophilic interactions. However, the catalytic efficiency of GO-rEstKp complex was lowered to approximately 40 % of that of free rEstKp. This study provides an insight into the addition of GO for industrial applications of cold-adapted enzymes at ambient temperatures.