- 作者：Lei Li ; Liya Wang ; Fang Le ; Xiaozhen Liu ; Ping Yu ; Jiangzhong Sheng ; Hefeng Huang ; Fan Jin
- 关键词：Assisted reproductive technology ; ART ; differentially methylated regions ; DMRs ; in vitro fertilization ; IVF ; twins
- 刊名：Fertility and Sterility
- 出版年：2011
- 出版时间：May 2011
- 年：2011
- 卷：95
- 期：6
- 页码：1975-1979
- 全文大小：174 K
Objective
To examine the effect of assisted reproductive technology on the stability of DNA methylation at differentially methylated regions (DMRs) in twins conceived by IVF.Design
Prospective clinical observational study.
Setting
IVF center, university-affiliated teaching hospital.
Patient(s)
Fifty-nine pairs of twins were recruited, including 29 pairs conceived through IVF and 30 pairs of naturally conceived twins.
Intervention(s)
Collection of umbilical cord blood samples.
Main Outcome Measure(s)
DNA was extracted from umbilical cord blood. Two maternally methylated regions (KvDMR1 and PEG1) and one paternally methylated region (H19/IGF2 DMR) were analyzed using bisulfite-based technologies.
Result(s)
Although H19/IGF2 DMR and KvDMR1 showed slightly more variable levels of methylation in IVF cases than in spontaneous cases, methylation indices did not reveal significant differences at three DMRs between IVF-conceived and naturally conceived twins.
Conclusion(s)
Our results suggest no significant increase in imprint variability at these DMRs, but the greater variance in the IVF twins has a biologically meaningful consequence and may be a topic for future investigation. Large cohorts are needed to systematically assess the potential epigenetic risk in twins conceived with IVF.