The dominant roles of ICAM-1-encoding gene in DNA vaccination against Japanese encephalitis virus are the activation of dendritic cells and enhancement of cellular immunity

详细信息    查看全文

• 作者：Yong-Zhen Zhai ; Yan Zhou ; Li Ma ; Guo-He Feng ; ^{guohefengcn@163.com}
• 关键词：APC ; antigen-presenting cell ; CFSE ; 5-(and 6-)carboxyfluorescein diacetate succinimidyl ester ; CTL ; cytotoxic T lymphocyte ; DC ; dendritic cell ; FBS ; fetal bovine serum ; FCS ; fetal calf serum ; FITC ; fluorescein isothiocyanate ; ICAM ; intercellular adhesion
• 刊名：Cellular Immunology
• 出版年：2013
• 出版时间：January, 2013
• 年：2013
• 卷：281
• 期：1
• 页码：1-10
• 全文大小：1448 K

文摘

We investigated the cellular immune responses elicited by a plasmid DNA vaccine encoding prM-E protein from the Japanese encephalitis (JE) virus (JEV) with or without various forms of intercellular adhesion molecule (ICAM)-1 gene to maximize the immune responses evoked by the JE DNA vaccine. We observed that co-immunization with the construct containing murine ICAM-1 gene (pICAM-1) resulted in a significant increase in the percentage of CD4⁺T cells, high level of JEV-specific cytotoxic T lymphocyte response, and high production of T helper 1 (Th1)-type cytokines in splenic T cells. Furthermore, the co-expression of ICAM-1 and DNA immunogens was found to be more effective in generating T cell-mediated immune responses than those induced by immunization with pJME in combination with pICAM-1. Our results suggested that ICAM-1 enhanced T cell receptor signaling and activated Th1 immune responses in the JEV model system by increasing the induction of CD4⁺Th1 cell subset and activating dendritic cells.