Carbohydrate-binding motifs in a novel type lectin from the sea mussel Crenomytilus grayanus: Homology modeling study and site-specific mutagenesis
详细信息 查看全文
- 作者：Svetlana N. Kovalchuka ; s.n.kovalchuk@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Vasily A. Golotina ; b ; golotin@bk.ru" class="auth_mail" title="E-mail the corresponding author ; Larissa A. Balabanovaa ; b ; balaban@piboc.dvo.ru" class="auth_mail" title="E-mail the corresponding author ; Nina S. Buinovskayaa ; ninok1993@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Galina N. Likhatskayaa ; galinlink@piboc.dvo.ru" class="auth_mail" title="E-mail the corresponding author ; Valery A. Rasskazova ; raskaz@piboc.dvo.ru" class="auth_mail" title="E-mail the corresponding author
- 关键词：GalNAc/gal-specific lectin ; Crenomytilus grayanus ; Carbohydrate-binding site ; Homology modeling ; Site-specific mutagenesis ; Hemagglutinating activity ; Mucin-binding activity
- 刊名：Fish & Shellfish Immunology
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：47
- 期：1
- 页码：565-571
- 全文大小：1237 K
文摘
- •
Structure of the CGL lectin belonging to a novel lectin family was studied.
- •
Homology modeling was used to predict sugar-binding sites of CGL.
- •
CGL has three sugar-binding sites with conserved HPY(K)G motifs.
- •
Effects of mutations in three HPY(K)G motifs on CGL activity were studied.
- •
All three HPY(K)G motifs are involved in CGL-carbohydrate interactions.