Human papillomavirus L1 protein expressed in Escherichia coli self-assembles into virus-like particles that are highly immunogenic
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- 作者：Yumei Chena ; b ; Yunchao Liub ; Gaiping Zhanga ; b ; c ; zhanggaiping2003@163.com" class="auth_mail" title="E-mail the corresponding author ; Aiping Wangd ; pingaw@126.com" class="auth_mail" title="E-mail the corresponding author ; Ziming Donga ; dongzm@zzu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yanhua Qid ; Jucai Wangc ; Baolei Zhaoc ; Ning Lid ; Min Jiangd
- 关键词：Human papillomavirus ; Bacterial expression ; Virus-like particles ; Splenic lymphocytes ; Neutralizing antibody
- 刊名：Virus Research
- 出版年：2016
- 出版时间：15 July 2016
- 年：2016
- 卷：220
- 期：Complete
- 页码：97-103
- 全文大小：1571 K
文摘
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We generated a native N-terminus of HPV 16 L1 protein from E. coli.
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SUMO tag improves the solubility expression of HPV L1 protein effectively.
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The HPV 16 L1 protein from E. coli can self-assemble into the VLPs in vitro.
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The HPV VLPs show morphology similar native HPV virions, with hemagglutinating activity.
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The VLPs elicit significant humoral and cellular immunity response against HPV.