Protective effects of the roots of Angelica sinensis on strenuous exercise-induced sports anemia in rats

详细信息    查看全文

• 作者：Chih-Wei Chang^a ; ^{wxes9050304@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Yi-Ming Chen^b ; ¹ ; Yi-Ju Hsu^b ; ¹ ; Chi-Chang Huang^b ; Yu-Tse Wu^a ; ^{ytwu@kmu.edu.tw" class="auth_mail" title="E-mail the corresponding author} ; Mei-Chich Hsu^c ; ^{meichich@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ALP ; alkaline phosphatase ; ALT ; alanine aminotransferase ; AS ; the roots of Angelica sinensis ; ASG ; strenuous exercise and treated with AS extract group ; AST ; aspartate aminotransferase ; BAT ; brown adipose tissue ; BUN ; blood urea nitrogen ; CAT ; catalase ; CG ; control group ; CK ; creatine kinase ; EDTA ; ethylenediaminetetraacetic acid ; EG ; strenuous exercise group ; EGTA ; ethylene glycol tetraacetic acid ; ERK ; extracellular signal-regulated kinase pathways ; H2O2 ; dihydrogen dioxide ; HCT ; hematocrit ; H
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：169-178
• 全文大小：3131 K

文摘

Sports anemia is a persistent and severe problem in athletes owing to strenuous exercise-induced oxidative stress and hepcidin upregulation. The roots of Angelica sinensis (AS), a familiar traditional Chinese medicine, has been used for replenishing blood since antiquity.

Aim of the study

To evaluate the effects of ethanolic AS extract in a 4-week study on sports anemia in female Wistar rats.

Materials and methods

To induce anemia, a strenuous exercise protocol consisting of running and swimming was employed with increasing intensity. Animals were randomly assigned to the following groups: control group; strenuous exercise group; and strenuous exercise and AS extract-treated group (300 mg kg⁻¹ d⁻¹). After 4 weeks, rats underwent exhaustive swimming and forelimb grip strength test. The blood biochemical markers and hepatic antioxidant activities were determined. Hepatic interleukin-6 and muscle glycogen were observed through immunohistochemical and Periodic acid–Schiff staining, respectively.

Results

AS extract (consisting of ferulic acid, Z-ligustilide, and n-butylidenephthalide) treatment improved forelimb grip strength and rescued exercise-induced anemia by significantly elevating the red blood cell counts and hemoglobin concentrations as well as hematocrit levels (p<0.05). AS modulated the iron metabolism through decreasing serum hepcidin-25 concentrations by 33.0% (p<0.05) and increasing serum iron levels by 34.3% (p<0.01). The hepatic injury marker serum alanine aminotransferase concentrations were also reduced, followed by increased antioxidant enzyme catalase expression in the liver (p<0.05). Furthermore, substantial attenuation of hepatic interleukin-6 expression and preservation of muscle glycogen content suggested the additional roles of AS acting on sports anemia and physical performance.

Conclusion

Our findings evidenced a novel and promising therapeutic approach for AS treatment for rescuing the anemic condition induced following 4 weeks of strenuous exercise.