Rapid loss of dendritic cell and monocyte responses to TLR ligands following venipuncture
详细信息 查看全文
- 作者：Angela Meier ; Amelia Fisher ; Harlyn K. Sidhu ; Judy J. Chang ; Tom F. Wen ; Hendrik Streeck ; Galit Alter ; Guido Silvestri ; Marcus Altfeld
- 关键词：Innate immunity ; Antigen presenting cells ; Dendritic cells ; Monocytes ; Toll like receptors ; Vaccine trials
- 刊名：Journal of Immunological Methods
- 出版年：2008
- 出版时间：31 December 2008
- 年：2008
- 卷：339
- 期：2
- 页码：132-140
- 全文大小：2083 K
文摘
Blood samples from multiple sites are collected in multicenter trials, and frequently shipped to centralized laboratories for processing and comparable experimental evaluation. It is therefore of crucial interest to assess the preservation of immune cell functions after overnight shipment of whole blood. Here we evaluated the ability of pDCs, mDCs and monocytes to respond to TLR ligands at multiple timepoints following venipuncture as compared to immediate processing. Our results demonstrate a profound impairment of APC function, in particular of IFN-α production of pDCs, if whole blood was processed later than 6 h after venipuncture. Overnight shipment or extended rest of whole blood before processing therefore severely compromises the ability of APCs to respond to TLR ligands, and this has to be taken into consideration when designing multicenter trials.