Investigation on the transcription factors of porcine 3¦Â-hydroxysteroid dehydrogenase and 17¦Â-hydroxysteroid dehydrogenase genes

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• 作者：Xinxing Dong^a ; Ying Bai^a ; Yi Xin^a ; Qinqin Xu^a ; Gang Chen^b ; ^{chengang02@yahoo.com} ; Meiying Fang^a ; ^{meiying@cau.edu.cn}
• 关键词：Pig ; 3¦Â-Hydroxysteroid dehydrogenase ; 17¦Â-Hydroxysteroid dehydrogenase ; Transcription factor
• 刊名：Gene
• 出版年：2012
• 出版时间：10 May, 2012
• 年：2012
• 卷：499
• 期：1
• 页码：186-190
• 全文大小：357 K

文摘

The enzymes 3¦Â-hydroxysteroid dehydrogenase (3¦ÂHSD) and 17¦Â-hydroxysteroid dehydrogenase (17¦ÂHSD) regulate the steroid metabolism in mammals. In this study, we aimed to characterize the steroid related transcription factors at the 5?flanking region of these two genes. A series of 5?deletions of approximately 1 kb of 5?flanking region on both genes were fused to a pGL3 basic vector containing firefly luciferase cDNA, and then transfected to human hepatocellular liver carcinoma cell line (HepG2). Luciferase activity assay indicated the region from ?#xA0;574 to ?#xA0;617 bp of the 3¦ÂHSD1 promoter, and from ?#xA0;850 to ?#xA0;868 bp of 17¦ÂHSD7 promoter induced the highest luciferase activity. A putative transcription factor, i.e. the proline and acidic amino acid-rich basic leucine zipper (PAR/bZIP) family of 3¦ÂHSD1 gene, and three-amino acid loop extension (TALE) homeodomain class of 17¦ÂHSD7 were identified respectively by sequence homology. Gel shift assay further confirmed the binding capacity of the putative elements to nuclear extract. Our study gives new insights to the transcriptional regulation of 3¦ÂHSD1 and 17¦ÂHSD7 and further hints to their involvement in steroid metabolism.