Housekeeping genes as internal standards: use and limits
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- 作者：Thellin ; O. ; Zorzi ; W. ; Lakaye ; B. ; De Borman ; B. ; Coumans ; B. ; Hennen ; G. ; Grisar ; T. ; Igout ; A. ; et. al.
- 关键词：Internal standards ; Housekeeping genes ; RNase protection ; RT-PCR ; G3PDH ; glyceraldehyde-3-phosphate dehydrogenase ; HPRT ; hypoxantine phosphoribosyltransferase ; PMA ; phorbol 10-myristate 13-acetate
- 刊名：Journal of Biotechnology
- 出版年：1999
- 出版时间：October 8, 1999
- 年：1999
- 卷：75
- 期：2-3
- 页码：291-295
- 全文大小：73.0 K
文摘
Quantitative studies are commonly realised in the biomedical research to compare RNA expression in different experimental or clinical conditions. These quantifications are performed through their comparison to the expression of the housekeeping gene transcripts like glyceraldehyde-3-phosphate dehydrogenase (G3PDH), albumin, actins, tubulins, cyclophilin, hypoxantine phosphoribosyltransferase (HRPT), L32. 28S and 18S rRNAs are also used as internal standards. In this paper, it is recalled that the commonly used internal standards can quantitatively vary in response to various factors. Possible variations are illustrated using three experimental examples. Preferred types of internal standards are then proposed for each of these samples and thereafter the general procedure concerning the choice of an internal standard and the way to manage its used are discussed.