Prospective analysis of 61 cases of enteroviral meningitis: interest of systematic genome detection in cerebrospinal fluid irrespective of cytologic examination results

详细信息    查看全文

• 作者：Henquell ; Cé ; cile ; Chambon ; Martine ; Bailly ; Jean-Luc ; Alcaraz ; Sté ; phanie ; De Champs ; Christophe ; et. al.
• 关键词：Enterovirus ; Meningitis ; Clinical diagnosis ; Genome detection ; PCR ; Cerebrospinal fluid ; CSF ; cerebrospinal fluid ; PCR ; polymerase chain reaction ; WCC ; white cell count
• 刊名：Journal of Clinical Virology
• 出版年：2001
• 出版时间：April, 2001
• 年：2001
• 卷：21
• 期：1
• 页码：29-35
• 全文大小：79.3 K

文摘

Background: Enteroviruses are the most commonly identified cause of viral meningitis. Detection of the enterovirus genome in cerebrospinal fluid (CSF) using reverse-transcription polymerase chain reaction (PCR) has proved to be useful in diagnosis and is more rapid and sensitive than viral cultures. In routine practice, cytologic examination results of CSF are obtained swiftly and PCR indication is performed as a second step. Objectives: The aim of this study was to determine, by analysis of complete data from CSF results for 61 cases of proven enteroviral meningitis, whether cytologic CSF findings can be used to establish viral etiology and to indicate if PCR assay should be performed. Study design: From a prospective study of children admitted during 1997 for suspected enterovirus meningitis in which PCR and viral cultures of CSF were systematically performed, we selected 61 patients with proven enterovirus meningitis. We compared global white cell count (WCC), relative percentage of lymphocytes/neutrophils, PCR and culture for enterovirus, patient age, and clinical data. Results: 92 % of patients (56/61) had positive PCR in CSF and in 48 % (29/61) enterovirus was isolated in CSF. Nine patients (14.75 % ) had WCC<10/mm³; eight of them had positive PCR and two had positive culture. There were comparable numbers of CSF with a predominance of lymphocytes (n