Heparin hydrogel sandwich cultures of primary hepatocytes
详细信息 查看全文
- 作者：Elena Foster ; Jungmok You ; Christian Siltanen ; Dipali Patel ; Amranul Haque ; Leif Anderson ; Alexander Revzin ; arevzin@ucdavis.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Heparin hydrogel ; 3D polymer matrix ; Hepatocyte ; Sandwich culture ; Polarity ; Hepatocyte growth factor
- 刊名：European Polymer Journal
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：72
- 期：Complete
- 页码：726-735
- 全文大小：2133 K
文摘
- •
Heparin-containing hydrogel used as sandwich-type scaffold for cultivation of functional primary hepatocytes.
- •
Gel layers fabricated via UV-induced thiol-ene coupling reaction of thiolated heparin (Hep-SH) and diacrylated poly(ethylene glycol) (PEG-DA) precursors.
- •
Heparin-binding hepatocyte growth factor (HGF) was retained in gel and promoted long-term cell functionality.
- •
Gel sandwich proven an excellent system for maintaining differentiated, polarized primary hepatocytes for over 3 weeks.