Detection and chromatographic removal of lipopolysaccharide in preparations of multifunctional galectins
详细信息 查看全文
- 作者：Kerstin Sarter ; Sabine André ; Herbert Kaltner ; Martin Lensch ; Connie Schulze ; Vilma Urbonaviciute ; Georg Schett ; Martin Herrmann ; Hans-Joachim Gabius
- 关键词：Adhesin ; Dendritic cell ; Lectin ; Lipopolysaccharide ; NF-κ ; B activation ; Tumor necrosis factor-α
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2009
- 出版时间：30 January 2009
- 年：2009
- 卷：379
- 期：1
- 页码：155-159
- 全文大小：225 K
文摘
The functional spectrum of human galectins is currently explored, with a wide range of activities being described. The role of galectin-3 as adhesin for bacteria is based on its strong binding to lipopolysaccharides (LPSs), which brings the possibility of such a contamination in galectin preparations to awareness. This assumption was verified in three independent functional assay systems using polymyxin B as inhibitor of LPS-dependent effects. Moreover, a commercial LPS quantification kit also revealed LPS in galectin preparations. Chromatography was effective in removing LPS, suggesting that such a technique needs to be applied to prevent assigning cellular responses to galectins rather than LPS.