Determination of substitution positions in hyaluronic acid hydrogels using NMR and MS based methods
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- 作者：Frida J. Wendea ; Frida.Wende@slu.se" class="auth_mail" title="E-mail the corresponding author ; Suresh Gohila ; Suresh.Gohil@slu.se" class="auth_mail" title="E-mail the corresponding author ; Hotan Mojarradib ; Hotan.Mojarradi@galderma.com" class="auth_mail" title="E-mail the corresponding author ; Thibaud Gerfaudc ; Thibaud.Gerfaud@galderma.com" class="auth_mail" title="E-mail the corresponding author ; Lars I. Nordb ; Lars.Nord@galderma.com" class="auth_mail" title="E-mail the corresponding author ; Anders Karlssonb ; Anders.Karlsson@galderma.com" class="auth_mail" title="E-mail the corresponding author ; Jean-Guy Boiteauc ; Jean-Guy.Boiteau@galderma.com" class="auth_mail" title="E-mail the corresponding author ; Anne Helander Kenneb ; Anne.HelanderKenne@galderma.com" class="auth_mail" title="E-mail the corresponding author ; Corine Sandströ ; ma ; Corine.sandstrom@slu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：HA ; hyaluronic acid/hyaluronan ; BDDE ; 1 ; 4-butanediol diglycidyl ether ; BDPE ; 1 ; 4-butanediol di-(propan-2 ; 3-diolyl)ether ; DCM ; dichloromethane ; THF ; tetrahydrofurane ; TOCSY ; total correlation spectroscopy ; HSQC ; heteronuclear single quantum correlation ; NOESY ; nuclear Overhauser effect spectroscopy ; HMBC ; heteronuclear multiple bond correlation ; VCD ; Vibrational circular dichroism
- 刊名：Carbohydrate Polymers
- 出版年：2016
- 出版时间：20 January 2016
- 年：2016
- 卷：136
- 期：Complete
- 页码：1348-1357
- 全文大小：1791 K
文摘
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NMR and MS methods to determine linker position in HA hydrogels were developed.
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Oligosaccharides of different sizes were obtained by enzymatic degradation.
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Substitution occur at all four available hydroxyl groups of the HA repeating unit.
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Linker distribution among the four positions was obtained by NMR and MS.
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Distribution of substitution positions depend on the size of the oligosaccharides.